Journal: Scientific Reports

Article Title: Targeting colony stimulating factor-1 receptor signalling to treat ectopic pregnancy

doi: 10.1038/s41598-020-72785-y

Figure Lengend Snippet: CSF-1R and CSF-1 expression in immortalised first trimester trophoblast cells (SW.71) ( A ) Representative two-dimensional confocal image of SW.71 trophoblast cells immunostained for CSF-1 ligand (green) and nuclei (blue). (B) Three-dimensional cross-section image of the SW.71 cell showing localisation of CSF-1 protein in the cytoplasm (CP). (C) Representative two-dimension confocal image of trophoblast cells immunostained for CSF-1R (green) and nuclei (blue) in the SW.71 cells. (D) Three-dimensional cross-section image of the SW.71 cell showing localisation of CSF-1R in the cell membrane (CM) (Inset) Negative control SW.71 cells with DAPI staining on the nuclei (N) and no immunoreactivity observed. Scale bars A/B/Inset 20 µm and BD 10 µm.

Article Snippet: Blocking was carried out with BSA and normal goat serum for 1 h. CSF-1 and CSF-1R were immunolocalised using primary antibodies conjugated with Alexa Fluor 488 against CSF-1 (Santa Cruz Biotechnology, Texas, USA, sc365779, 1:500) and CSF-1R (Santa Cruz Biotechnology, Texas, USA, sc365719, 1:500) overnight at 4 °C with blocking peptide as a negative control.

Techniques: Expressing, Membrane, Negative Control, Staining

Journal: Scientific Reports

Article Title: Targeting colony stimulating factor-1 receptor signalling to treat ectopic pregnancy

doi: 10.1038/s41598-020-72785-y

Figure Lengend Snippet: Effect of CSF-1 and CSF-1R antagonism with GW2580 on trophoblast cell proliferation, migration and viability (A) Effects of exogenous CSF-1 (50–200 ng/ml) on SW.71 cell proliferation after 48 h exposure. (B) Effects of GW2580 (20 and 40 µM) on proliferation as a percentage of vehicle control after 48 h. (C) Effects of exogenous CSF-1 (100 ng/ml) with GW2580 (5–40 µM) on as a percentage of vehicle control after 48 h. (D) Effect of CSF-1 (100 ng/ml) and GW2580 (20–40 µM) on viable cell number as a percentage of control. (E) The effect of 40 µM GW2580 in the presence or absence of CSF-1 (100 ng/ml) of cell cytotoxicity. (F) A representative image showing scratch imaged at 0 h and at 18 h using TScratch software. (G) Effects of GW2580 (20–40 µM) and exogenous CSF-1 ligand (100 ng/ml) on SW.71 cellular migration after an 18 h incubation. Vehicle (DMSO) treated cells were used as controls and values normalised to the control. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Blocking was carried out with BSA and normal goat serum for 1 h. CSF-1 and CSF-1R were immunolocalised using primary antibodies conjugated with Alexa Fluor 488 against CSF-1 (Santa Cruz Biotechnology, Texas, USA, sc365779, 1:500) and CSF-1R (Santa Cruz Biotechnology, Texas, USA, sc365719, 1:500) overnight at 4 °C with blocking peptide as a negative control.

Techniques: Migration, Control, Software, Incubation

Journal: Scientific Reports

Article Title: Targeting colony stimulating factor-1 receptor signalling to treat ectopic pregnancy

doi: 10.1038/s41598-020-72785-y

Figure Lengend Snippet: Immunolocalisation of CSF-1R and CSF-1 in tubal EP implantation sites. (A ) Representative haematoxylin and eosin image of the ectopic implantation site in the Fallopian tube. (B) Negative control DAPI stained section (blue) showing no CSF-1R staining (red). (C) Section of human Fallopian tube ectopic implantation site immunostained for CSF-1R showing staining (red) and confirming the expression of CSF-1R. (D , E) Representative image showing immunolocalisation of CSF-1 in trophoblast invading a human FT. Immunolocalisation of CSF-1 at ectopic implantation sites is observed as green staining. (F) CSF-1 immunostaining (green staining highlighted by white arrows) with DAPI (blue) staining the nuclei of trophoblasts at ectopic implantation site. (G) negative control section with DAPI (blue) staining. (H , I) Representative image showing immunolocalisation (green) of CSF-1R in trophoblast invading a human FT. (J) Immunolocalisation of CSF-1R on ectopic implantation sites is shown by green staining (white arrows) with DAPI (blue) staining nuclei of trophoblasts at ectopic implantation site. (K) No immunostaining in control section with DAPIU (blue staining). FT Fallopian tube, CTB cytotrophoblast, STB syncytiotrophoblast. Scale bars represent 100 µm.

Article Snippet: Blocking was carried out with BSA and normal goat serum for 1 h. CSF-1 and CSF-1R were immunolocalised using primary antibodies conjugated with Alexa Fluor 488 against CSF-1 (Santa Cruz Biotechnology, Texas, USA, sc365779, 1:500) and CSF-1R (Santa Cruz Biotechnology, Texas, USA, sc365719, 1:500) overnight at 4 °C with blocking peptide as a negative control.

Techniques: Negative Control, Staining, Expressing, Immunostaining, Control

Journal: Frontiers in Microbiology

Article Title: A Novel Assay for Detection of Methicillin-Resistant Staphylococcus aureus Directly From Clinical Samples

doi: 10.3389/fmicb.2020.01295

Figure Lengend Snippet: Assay specificity in various strains of coagulase-negative staphylococci and non-staphylococcal bacteria.

Article Snippet: Oligella ureolytica , ATCC 43534 , – , – , Not MRSA.

Techniques: Bacteria